New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00359446" target="_blank" >RIV/60077344:_____/11:00359446 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/11:43882557 RIV/60076658:12220/11:43882557
Result on the web
<a href="http://dx.doi.org/10.1016/j.vetpar.2010.11.010" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2010.11.010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vetpar.2010.11.010" target="_blank" >10.1016/j.vetpar.2010.11.010</a>

Result language
angličtina
Original language name
New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II
Original language description
Two species of Cryptosporidium are commonly identified in pigs: C. suis and C. pig genotype II. Detection is routinely based on molecular methods such as PCR and subsequent RFLP or gene sequencing. Low sensitivity to mixed infections is solved by designing and testing novel species-specific primers in the present study. Sensitivity of our primers was identical to genus-specific primers, but more (1:48) mixed infections were detected using these species-specific primers on 477 DNA samples originating from naturally infected pigs of different age categories. Our results show differences in age-dependent susceptibility of pigs to the infection. Whereas C. suis was found in all tested categories of pigs (1-12 week of age and sows). Cryptosporidium pig genotype II was recorded only in animals older than 6 week of age. Usage of species-specific primers could help to better the understanding of epidemiology and occurrence (often underestimated) of pig specific Cryptosporidium spp.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
—

Project
<a href="/en/project/GP523%2F07%2FP117" target="_blank" >GP523/07/P117: Zoonotic potential of Cryptosporidium species infecting cattle and pigs</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)