Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00362689" target="_blank" >RIV/60077344:_____/11:00362689 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/11:43882917
Result on the web
<a href="http://dx.doi.org/10.1073/pnas.1107060108" target="_blank" >http://dx.doi.org/10.1073/pnas.1107060108</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1107060108" target="_blank" >10.1073/pnas.1107060108</a>
Alternative languages
Result language
angličtina
Original language name
Hyperprolinemic larvae of the drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen
Original language description
The larva of the drosophilid fly, Chymomyza costata, is probably the most complex metazoan organism that can survive submergence in liquid nitrogen (-196°C) in a fully hydrated state. We examined the associations between the physiological and biochemicalparameters of differently acclimated larvae and their freeze tolerance. Profiling of 61 different metabolites identified proline as a prominent compound whose concentration increased from 20 to 147 mM during diapause transition and subsequent cold acclimation. This study provides direct evidence for the essential role of proline in high freeze tolerance. Differential scanning calorimetry analysis suggested that high proline levels, in combination with a relatively low content of osmotically active water and freeze dehydration, increased the propensity of the remaining unfrozen water to undergo a glass-like transition (vitrification) and thus facilitated the prevention of cryoinjury.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
108
Issue of the periodical within the volume
32
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
13041-13046
UT code for WoS article
000293691400023
EID of the result in the Scopus database
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