Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00364474" target="_blank" >RIV/60077344:_____/11:00364474 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/11:00364474
Result on the web
<a href="http://dx.doi.org/10.1159/000327716" target="_blank" >http://dx.doi.org/10.1159/000327716</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1159/000327716" target="_blank" >10.1159/000327716</a>
Alternative languages
Result language
angličtina
Original language name
Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization
Original language description
The Western Palearctic water frogs Pelophylax ridibundus and P. lessonae were identified as parental (sexual) species and P. esculentus as their interspecific, hybridogenetically reproducing hybrid with hemiclonal heredity. We used genomic in situ hybridization (GISH) to identify parental chromosomes of P. lessonae and P. ridibundus in diploid P. esculentus karyotypes (2n = 26). GISH probes were made by fluorochrome labeling of total genomic DNA extracted from the sexual progenitors. The labeled probe from one species was hybridized to chromosomes of P. esculentus in the presence of excess of unlabeled genomic DNA from the other species. Thus, the P. lessonae probe was blocked by P. ridibundus unlabeled DNA, and vice versa. We successfully discriminated each of the 13 respective parental chromosomes in metaphase complements of the hybrids according to species-specific hybridization signals.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytogenetics and Genome Research
ISSN
1424-8581
e-ISSN
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Volume of the periodical
134
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
7
Pages from-to
206-212
UT code for WoS article
000292469000006
EID of the result in the Scopus database
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