Molecular identification and genotyping of Microsporidia in selected hosts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00375252" target="_blank" >RIV/60077344:_____/12:00375252 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00436-011-2543-9" target="_blank" >http://dx.doi.org/10.1007/s00436-011-2543-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00436-011-2543-9" target="_blank" >10.1007/s00436-011-2543-9</a>

Result language
angličtina
Original language name
Molecular identification and genotyping of Microsporidia in selected hosts
Original language description
The work is described by microscopic, serological (IFAT) and molecular analysis of isolates from clinical samples (blood, faeces and urine) from ten domestic rabbits (Oryctolagus cuniculus), breed MalikA1/2, four New Zealand domestic rabbits, 11 sows ofbreed Slo0076akian Improved White and 15 clinically healthy laboratory BALB/c mice. The aim of the study was to validate the suitability of species-unspecific primer pairs 530F and 580R for genotype determination of the Microsporidia strain and species-specific primer pairs ECUNF and ECUNR, SINTF and SINTR and EBIER1 and EBIEF1 for the determination of Encephalitozoon cuniculi, E. intestinalis and Enterocytozoon bieneusi species for diagnostic purposes. Sequences of animals were compared with those fromthe GenBank database. In rabbits, two murine genotypes II and four canine genotypes III were identified. Genotype II was identified in mice. The E. intestinalis identified in the sample from swine showed no genetic heterogeneity.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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