Wolbachia and DNA barcoding Insects: Patterns, potential, and problems

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00378011" target="_blank" >RIV/60077344:_____/12:00378011 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/12:43883570
Result on the web
<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036514" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036514</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0036514" target="_blank" >10.1371/journal.pone.0036514</a>

Result language
angličtina
Original language name
Wolbachia and DNA barcoding Insects: Patterns, potential, and problems
Original language description
Wolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts and can confuse the patterns of mitochondrial variation, including DNA barcodes. We examined the extent to which is Wolbachia detected in DNA barcoding, assessed its impact upon identification accuracy, and considered the variation present in Wolbachia COI. When two million insect COI trace files were examined on the Barcode of Life Datasystem, Wolbachia COI was present in 0.16% of the cases. It is possibleto generate Wolbachia COI using standard insect primers, but that amplicon was never confused with host COI. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. The presence of theWolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library. Regular assays for Wolbachia presence and type should be adopted by large scale insect barcoding initiatives.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
<a href="/en/project/GAP505%2F10%2F0673" target="_blank" >GAP505/10/0673: Inter-specific interactions in complex food webs of tropical rain forests: a whole-forest experimental approach</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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