Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00380582" target="_blank" >RIV/60077344:_____/12:00380582 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/12:00380582

Result on the web

<a href="http://home.ueb.cas.cz/publikace/2012_Navratil_IOBC_61-67.pdf" target="_blank" >http://home.ueb.cas.cz/publikace/2012_Navratil_IOBC_61-67.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

Original language description

Extracellular serine proteases are often used as a factor of pathogenicity. To improve potato resistance to the pathogens, we prepared plants containing a gene derived from the wax moth Galleria mellonella and expressing the silk protease inhibitor 2 (SPI2). The synthetic SPI2 gene was fused with mGFP-5 to facilitate its detection in plants. Cultivar Velox, which exhibits medium resistance to Phytophthora infestans, was used for transgenosis. The polyclonal antibody confirmed the presence of a protein of expected size in the extracts of transgenic plants. The presence of the fusion protein in resistant plants was confirmed with additional analytical methods. Indirect ELISA showed up to 3 fold increase of protease inhibition in transformed plants compared to the control background level. The tested resistance to late blight showed heterogeneity among transgenic lines, i.e. some lines were more resistant and others were more susceptible than the non-transgenic plants.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

IOBC-Wprs Bulletin

ISSN

1027-3115

e-ISSN

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Volume of the periodical

73

Issue of the periodical within the volume

Jan 2012

Country of publishing house

CH - SWITZERLAND

Number of pages

7

Pages from-to

61-67

UT code for WoS article

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EID of the result in the Scopus database

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