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Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00380582" target="_blank" >RIV/60077344:_____/12:00380582 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389030:_____/12:00380582

  • Result on the web

    <a href="http://home.ueb.cas.cz/publikace/2012_Navratil_IOBC_61-67.pdf" target="_blank" >http://home.ueb.cas.cz/publikace/2012_Navratil_IOBC_61-67.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

  • Original language description

    Extracellular serine proteases are often used as a factor of pathogenicity. To improve potato resistance to the pathogens, we prepared plants containing a gene derived from the wax moth Galleria mellonella and expressing the silk protease inhibitor 2 (SPI2). The synthetic SPI2 gene was fused with mGFP-5 to facilitate its detection in plants. Cultivar Velox, which exhibits medium resistance to Phytophthora infestans, was used for transgenosis. The polyclonal antibody confirmed the presence of a protein of expected size in the extracts of transgenic plants. The presence of the fusion protein in resistant plants was confirmed with additional analytical methods. Indirect ELISA showed up to 3 fold increase of protease inhibition in transformed plants compared to the control background level. The tested resistance to late blight showed heterogeneity among transgenic lines, i.e. some lines were more resistant and others were more susceptible than the non-transgenic plants.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    IOBC-Wprs Bulletin

  • ISSN

    1027-3115

  • e-ISSN

  • Volume of the periodical

    73

  • Issue of the periodical within the volume

    Jan 2012

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    7

  • Pages from-to

    61-67

  • UT code for WoS article

  • EID of the result in the Scopus database