Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00380704" target="_blank" >RIV/60077344:_____/12:00380704 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/12:43883622 RIV/00027162:_____/12:#0000925
Result on the web
<a href="http://link.springer.com/article/10.1007/s10709-012-9668-3" target="_blank" >http://link.springer.com/article/10.1007/s10709-012-9668-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10709-012-9668-3" target="_blank" >10.1007/s10709-012-9668-3</a>

Result language
angličtina
Original language name
Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae)
Original language description
The olive fruit fly, Bactrocera oleae, has a diploid set of 2n = 12 chromosomes including a pair of sex chromosomes, XX in females and XY in males, but polytene nuclei show only five polytene chromosomes, formed by five autosome pairs. We examined the fate of the sex chromosomes in the polytene complements of this species using FISH with the X and Y chromosome-derived probes, prepared by laser microdissection of the respective chromosomes from mitotic metaphases. In polytene nuclei, both probes hybridized strongly to a granular heterochromatic network. The X chromosome probe highlighted most of the granular mass, whereas the Y chromosome probe identified a small compact body of this network. We also examined distribution of rDNA using FISH with an 18SrDNA probe that hybridized exclusively to the sex chromosomes. Our study clearly shows that the granular heterochromatic network of polytene nuclei in B. oleae is formed by the underreplicated sex chromosomes and associated rDNA.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/IAA600960925" target="_blank" >IAA600960925: Evolutionary history of sex chromosomes in Lepidoptera</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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