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First Report of Hop stunt viroid Infecting Hop in Slovenia

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381533" target="_blank" >RIV/60077344:_____/12:00381533 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    First Report of Hop stunt viroid Infecting Hop in Slovenia

  • Original language description

    Since 2007, symptoms similar to Hop stunt viroid (HSVd) infection have been observed in several hop gardens in Slovenia. Symptoms include stunting, leaf curl, small cones, and dry root rot. In the first year, the incidence varied from 1 to 30% and increased rapidly (by as much as 10%) each subsequent year, mostly along plant rows. For molecular identification of the pathogen, RNA was extracted from leaves and cones of symptomatic and asymptomatic plants from two different hop gardens. Reverse transcription-PCR was carried out using two pairs of specific HSVd primers. Both primer pairs gave a specific PCR product from symptomatic plant tissue, but not from asymptomatic samples. PCR products were subjected to sequencing. Five sequences obtained had 96-99% sequence identity with various HSVd variants from GenBank. To our knowledge, this is the first report of HSVd on hop in Europe. Strict phytosanitary measures have been taken to prevent further spread and to eradicate HSVd infections.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GCP501%2F10%2FJ018" target="_blank" >GCP501/10/J018: Pospiviroid pathogenesis as ?regulatory disorder? mediated by viroid-specific small RNAs.</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů