Genetic diversity of Czech Candidatus Phytoplasma mali? strains based on multilocus gene analyses

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00395034" target="_blank" >RIV/60077344:_____/13:00395034 - isvavai.cz</a>
Alternative codes found
RIV/25271121:_____/13:#0000477
Result on the web
<a href="http://dx.doi.org/10.1007/s10658-013-0196-5" target="_blank" >http://dx.doi.org/10.1007/s10658-013-0196-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10658-013-0196-5" target="_blank" >10.1007/s10658-013-0196-5</a>

Result language
angličtina
Original language name
Genetic diversity of Czech Candidatus Phytoplasma mali? strains based on multilocus gene analyses
Original language description
During 2010, samples from several apple trees were collected throughout the CR to verify identity of phytoplasmas detected in association with the apple proliferation disease. The majority of the 74 apple trees examined using molecular tools were positive for Ca. P. mali?. The 16S - 23S ribosomal genes, the ribosomal protein genes and the nitroreductase and rhodonase like genes were then studied to verify phytoplasma strain variability on multigenic bases. Two RFLP profiles and correspondingly two genetic lineages were found in the PCR-amplified fragments covering the 16S-23S rDNA spacer region. Ca. P. mali? strains belonging to rpX-A subgroup were identified in the majority of the apple tree sampled. The apple proliferation subtypes AP-15 and AT-2 exhibited nearly equal occurrence; the AT-1 subtype and a mixture of the two or all three of the AP subtypes were infrequently found. The PCR/RFLP results were confirmed by nucleotide sequence analyses of selected Ca. P. mali? strains.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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