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In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00421276" target="_blank" >RIV/60077344:_____/13:00421276 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12310/13:43885518

  • Result on the web

    <a href="http://dx.doi.org/10.1111/tbed.12122" target="_blank" >http://dx.doi.org/10.1111/tbed.12122</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/tbed.12122" target="_blank" >10.1111/tbed.12122</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    In vitro Culture of a Novel Genotype of Ehrlichia sp from Brazil

  • Original language description

    Ehrlichiae are obligate intracytoplasmic Gram-negative, tick-borne bacteria belonging to the Anaplasmataceae family. Ehrlichioses are considered emerging diseases in both humans and animals. Several members of the genus Ehrlichia have been isolated and propagated in vitro. This study describes the continuous propagation of a Brazilian Ehrlichia sp. isolate in IDE8 tick cells, canine DH82 cells and bovine aorta cells. Initially, the organisms were isolated from the haemolymph of a Rhipicephalus (Boophilus) microplus tick into IDE8 cells. Infected IDE8 cells were brought from Brazil to Germany, where the organisms were continuously propagated in IDE8, DH82 and bovine aorta cells. Bovine aorta cells were infected and propagated for 3months, correspondingto six subcultures, whereas the other two infected cell lines were kept for more than 1year. During the cultivation period, 36 and 14 subcultures were carried out in IDE8 and DH82 cell cultures, respectively. Reinfection of IDE8 cells wit

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Transboundary and Emerging Diseases

  • ISSN

    1865-1674

  • e-ISSN

  • Volume of the periodical

    60

  • Issue of the periodical within the volume

    NOV 2013

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    7

  • Pages from-to

    86-92

  • UT code for WoS article

    000326839500013

  • EID of the result in the Scopus database