Estimating trematode prevalence in snail hosts using a single-step duplex PCR: how badly does cercarial shedding underestimate infection rates?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00430917" target="_blank" >RIV/60077344:_____/14:00430917 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/1756-3305-7-243" target="_blank" >http://dx.doi.org/10.1186/1756-3305-7-243</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1756-3305-7-243" target="_blank" >10.1186/1756-3305-7-243</a>

Result language

angličtina

Original language name

Estimating trematode prevalence in snail hosts using a single-step duplex PCR: how badly does cercarial shedding underestimate infection rates?

Original language description

Trematode communities often consist of different species exploiting the same host population, with two or more trematodes sometimes co-occuring in the same host. A commonly used diagnostic method to detect larval trematode infections in snails has been based on cercarial shedding, though it is often criticized as inaccurate. In the present study we compare infection prevalences determined by cercarial emission with those determined, for the first time, by molecular methods, allowing us to quantify the underestimation of single and double infections based on cercarial emission. We thus developed a duplex PCR for two host-parasite systems, to specifically differentiate between single and double infections. The Ebro samples include two morphologically similar opecoelids, whereas the Otago samples include two morphologically different larval trematodes.Overall infection prevalences determined by duplex PCR were consistently and substantially higher than those based on cercarial shedding: a

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - European Centre of Ichtyoparasitology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasites Vectors

ISSN

1756-3305

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

MAY 2014

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000338266600002

EID of the result in the Scopus database

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