Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00433365" target="_blank" >RIV/60077344:_____/14:00433365 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture
Original language description
In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2?3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50?60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Protection Science
ISSN
1212-2580
e-ISSN
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Volume of the periodical
50
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
5
Pages from-to
174-178
UT code for WoS article
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EID of the result in the Scopus database
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