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Biosynthesis of Colabomycin E, a New Manumycin-Family Metabolite, Involves an Unusual Chain-Length Factor

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00434468" target="_blank" >RIV/60077344:_____/14:00434468 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/14:00434468 RIV/68378050:_____/14:00434468 RIV/00023001:_____/14:00059042

  • Result on the web

    <a href="http://dx.doi.org/10.1002/cbic.201400068" target="_blank" >http://dx.doi.org/10.1002/cbic.201400068</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/cbic.201400068" target="_blank" >10.1002/cbic.201400068</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Biosynthesis of Colabomycin E, a New Manumycin-Family Metabolite, Involves an Unusual Chain-Length Factor

  • Original language description

    Colabomycin E is a new member of the manumycin-type metabolites produced by the strain Streptomyces aureus SOK1/5-04 and identified by genetic screening from a library of streptomycete strains. The structures of colabomycin E and accompanying congeners were resolved. The entire biosynthetic gene cluster was cloned and expressed in Streptomyces lividans. Bioinformatic analysis and mutagenic studies identified components of the biosynthetic pathway that are involved in the formation of both polyketide chains. Recombinant polyketide synthases (PKSs) assembled from the components of colabomycin E and asukamycin biosynthetic routes catalyzing the biosynthesis of "lower" carbon chains were constructed and expressed in S. aureus SOK1/5-04 Delta colC11-14 deletion mutant. Analysis of the metabolites produced by recombinant strains provided evidence that in both biosynthetic pathways the length of the lower carbon chain is controlled by an unusual chain-length factor supporting biosynthesis eit

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/NT13012" target="_blank" >NT13012: The effect of manumycin-related compounds on innate immunity cells and their interactions</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chembiochem

  • ISSN

    1439-4227

  • e-ISSN

  • Volume of the periodical

    15

  • Issue of the periodical within the volume

    9

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    12

  • Pages from-to

    1334-1345

  • UT code for WoS article

    000337638400016

  • EID of the result in the Scopus database