Validation of Flotac for the detection and quantification of Troglodytella abrassarti and Neobalantidium coli in chimpanzees and pigs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00438007" target="_blank" >RIV/60077344:_____/14:00438007 - isvavai.cz</a>

Alternative codes found

RIV/68081766:_____/14:00434089 RIV/62157124:16170/14:43872668 RIV/62157124:16810/14:43872668

Result on the web

<a href="http://dx.doi.org/10.1645/14-505.1" target="_blank" >http://dx.doi.org/10.1645/14-505.1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1645/14-505.1" target="_blank" >10.1645/14-505.1</a>

Result language

angličtina

Original language name

Validation of Flotac for the detection and quantification of Troglodytella abrassarti and Neobalantidium coli in chimpanzees and pigs

Original language description

We carried out a calibration of FLOTAC for ciliates Troglodytella abrassarti and Neobalantidium coli based on the selection of a most appropriate flotation solutions, and we also tested its accuracy (i.e., number of detected stages out of known added number of stages to fecal samples) and sensitivity for trophozoites of both ciliates in chimpanzee feces and N. coli cysts in pig feces, compared the detection threshold of FLOTAC with MIF-based sedimentation, and, subsequently, tested the losses of ciliatestages during sample preparation. Nine flotation solutions were evaluated, and ZnSO4 solution (specific gravity [s.g.] 1.2) showed to be the most suitable for trophozoite detection, while Sheather's solution (s.g. 1.33) was selected as most suitable forcysts. The FLOTAC sensitivity in detection of both stages varied: for trophozoites, we found all samples were positive when the intensity of infection 10 trophozoites per gram and higher, whereas for cysts the sensitivity was lower. The

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Parasitology

ISSN

0022-3395

e-ISSN

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Volume of the periodical

100

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

662-670

UT code for WoS article

000344159300015

EID of the result in the Scopus database

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