Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00444547" target="_blank" >RIV/60077344:_____/15:00444547 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3389/fmicb.2014.00765" target="_blank" >http://dx.doi.org/10.3389/fmicb.2014.00765</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2014.00765" target="_blank" >10.3389/fmicb.2014.00765</a>

Result language

angličtina

Original language name

Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1 epsilon subgroup

Original language description

Manure is known to contain residues of antibiotics administered to farm animals as well as bacteria carrying antibiotic resistance genes (ARGs). These genes are often located on mobile genetic elements. In biogas plants (BGPs), organic substrates such asmanure and plant material are mixed and fermented in order to provide energy, and resulting digestates are used for soil fertilization. The fate of plasmid carrying bacteria from manure during the fermentation process is unknown. The present study focused on transferable antibiotic resistance plasmids from digestates of seven BGPs, using manure as a co-substrate, and their phenotypic and genotypic characterization. Plasmids conferring resistance to either tetracycline or sulfadiazine were captured by means of exogenous plasmid isolation from digestates into Pseudomonas putida KT2442 and Escherichia coli CV601 recipients, at transfer frequencies ranging from 10(-5) to 10(-7). Transconjugants (n = 101) were screened by PCR-Southern blot

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/EE2.3.30.0032" target="_blank" >EE2.3.30.0032: Promotion of post-doctoral positions in the Biology Centre of the Czech Academy of Sciences aimed to build the global competitiveness of the research teams in the Czech Republic</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

January

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

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UT code for WoS article

000348859400001

EID of the result in the Scopus database

2-s2.0-84927518366