Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00448715" target="_blank" >RIV/60077344:_____/15:00448715 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00448715
Result on the web
<a href="http://dx.doi.org/10.3389/fmicb.2015.00814" target="_blank" >http://dx.doi.org/10.3389/fmicb.2015.00814</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmicb.2015.00814" target="_blank" >10.3389/fmicb.2015.00814</a>
Alternative languages
Result language
angličtina
Original language name
Evolution of cyclizing 5-aminolevulinate synthases in the biosynthesis of actinomycete secondary metabolites: outcomes for genetic screening techniques
Original language description
A combined approach, comprising PCR screening and genome mining, was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) in streptomycetes-related strains. In actinomycetes, these genes were believed to be directly connected with the production of secondary metabolites carrying the C5N unit, 2-amino-3-hydroxycyclopent-2-enone, with biological activities making them attractive for future use in medicine and agriculture. Unlike "classical" primary metabolism ALAS, the C5N unit-forming cyclizing ALAS (cALAS) catalyses intramolecular cyclization of nascent 5-aminolevulinate. Specific amino acid sequence changes can be traced by comparison of "classical" ALASs against cALASs. PCR screening revealed 226 hemA gene-carrying strains from 1,500 tested, with 87% putatively encoding cALAS. Phylogenetic analysis of the hemA homologs revealed strain clustering according to putative type of metabolic product, which could be used to
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LH12191" target="_blank" >LH12191: New manumycins by means of the specific genetic screening of natural streptomycete isolates and of the combinatorial biosynthesis</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Microbiology
ISSN
1664-302X
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
AUG 2015
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
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UT code for WoS article
000359485700002
EID of the result in the Scopus database
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