Bioenergetics of the Bloodstream T. brucei Mitochondrion: a New Look
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00488328" target="_blank" >RIV/60077344:_____/15:00488328 - isvavai.cz</a>
Result on the web
<a href="http://www.parazitologie.cz/protozoologie/Protodny2015/JPD_sbornik_2015.pdf" target="_blank" >http://www.parazitologie.cz/protozoologie/Protodny2015/JPD_sbornik_2015.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Bioenergetics of the Bloodstream T. brucei Mitochondrion: a New Look
Original language description
The infective bloodstream stage (BS) of Trypanosoma brucei possesses a single mitochondrion that lacks a cytochrome-mediated respiratory chain and thus employs the hydrolytic activity of FoF1-ATPase to maintain the essential mitochondrial (mt) membrane potential (m). Meanwhile, dyskinetoplastic (Dk) trypanosomes lacking the mt encoded A6 that is essential for the functional Fo proton pore alternatively maintain their m by combining the hydrolytic activity of the matrix-facing F1-ATPase and the electrogenic exchange of ATP4- for ADP3- by the ADP/ATP carrier (AAC). Interestingly, the EC50 values of AAC inhibitor atractyloside are approximately 100× higher in BS cells compared to EC50 values measured for Dk trypanosomes. This result would suggest that AAC activity is not as important for BS as for Dk cells and thus the ATP for maintaining the m in BS cells is provided by mt substrate phosphorylation pathway(s). Indeed, RNAi silencing of AAC in BS trypanosomes has no effect on growth in vitro. The alternative sources of mt ATP will be discussed and revisited mt energy metabolism map of the infectious stage of the parasite will be presented.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LL1205" target="_blank" >LL1205: Exploration of the unique charakters od the Trypanosoma brucei FoF1 ATP synthase complex for future drug development against african sleeping sickness.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů