Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00458358" target="_blank" >RIV/60077344:_____/16:00458358 - isvavai.cz</a>
Alternative codes found
RIV/00843989:_____/16:E0105395
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0021967316302692" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967316302692</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2016.03.019" target="_blank" >10.1016/j.chroma.2016.03.019</a>

Result language
angličtina
Original language name
Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry
Original language description
A novel heptafluorobutyl chloroformate reagent (HFBCF) was examined for in-situ derivatization of amino-carboxylic metabolites in human urine. The sample preparation protocol provides cleaner extracts than other urinary GC–MS based methods. Reaction products of 153 urinary metabolites with HFBCF and two internal standards were investigated in detail by GC–MS and LC–MS. The new method was validated for 132 metabolites in artificial urine with special attention to potential disease biomarker candidates. The sample preparation protocol was finally evaluated by means of a certified organic acid standard mixture in urine and by GC–MS analysis of 100 morning urines obtained from healthy patients, where 112 physiological metabolites were quantified in a 25 µL sample aliquot. Most metabolites were found within the range reported in the reference human metabolome (HMDB) database and literature. The described method has been a promising tool for targeted GC–MS based metabolomic analysis in urine.n
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/GA13-18509S" target="_blank" >GA13-18509S: New alkyl chloroformate labeling strategies for the targeted and comparative mass spectrometry-based metabolomics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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