Multigene Sequence Analysis of Aster Yellows Phytoplasma Associated with Primrose Yellows

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00459073" target="_blank" >RIV/60077344:_____/16:00459073 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/jph.12442" target="_blank" >http://dx.doi.org/10.1111/jph.12442</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jph.12442" target="_blank" >10.1111/jph.12442</a>

Result language

angličtina

Original language name

Multigene Sequence Analysis of Aster Yellows Phytoplasma Associated with Primrose Yellows

Original language description

Primula acaulis (L.) Hill. plants showing stunting, leaf-yellowing and virescence were first discovered in the Czech Republic. Polymerase chain reactions with subsequent restriction fragment length polymorphism analyses and sequencing enabled classification of the detected phytoplasmas into the aster yellows group, ribosomal subgroup 16SrI-B, tufI-B, rpI-B, groELIB-III and SecY-IB subgroups. Phylogeny of the 16S rRNA gene sequences as well as sequence analysis of several chromosomal regions, such as the 16S-23S ribosomal operon, ribosomal proteins, spc ribosomal protein operon, genes for elongation factor EF-Tu, molecular chaperonin large subunit GroEL, immunodominant membrane protein, ribosome recycling factor, urydilate kinase, ATP- and Zn2+-dependent proteases not only confirmed its affiliation with the Candidatus Phytoplasma asteris' species but also enabled its detailed molecular characterization. The less researched regions of phytoplasma genome (amp, adk, hflB, pyrH-frr genes) could be valuable as additional markers for phytoplasma through differentiation especially within the 16SrI-B ribosomal subgroup.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Phytopathology - Phytopathologische Zeitschrift

ISSN

0931-1785

e-ISSN

—

Volume of the periodical

164

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

166-176

UT code for WoS article

000370141200003

EID of the result in the Scopus database

2-s2.0-84958957303