Multigene characterization of a new 'Candidatus Phytoplasma rubi'-related strain associated with blackberry witches' broom
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00464745" target="_blank" >RIV/60077344:_____/16:00464745 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1099/ijsem.0.000871" target="_blank" >http://dx.doi.org/10.1099/ijsem.0.000871</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/ijsem.0.000871" target="_blank" >10.1099/ijsem.0.000871</a>
Alternative languages
Result language
angličtina
Original language name
Multigene characterization of a new 'Candidatus Phytoplasma rubi'-related strain associated with blackberry witches' broom
Original language description
A new phytoplasma was identified in naturally infected blackberry plants exhibiting witches' broom symptoms in Portugal. The 16S rRNA gene sequence revealed that it is related to 'Candidatus Phytoplasma rubi' (16SrV-E ribosomal subgroup) and RFLP analysis revealed a unique profile following Mse I endonuclease digestion of R16F2n/R2 amplicons that distinguished it from the strains belonging to previously established 16SrV phytoplasma subgroups. The in silico restriction analyses confirmed that the phytoplasma strain from blackberry is different from all the other strains reported in group 16SrV. Phylogeny of the 16S rRNA gene sequences, sequence analyses of 16S-23S, tuf, rplV-rpsC, rplF-rplR, rplO-SecY-map and uvrB-degV genetic loci, as well as the variability of unique oligonucleotide sequences defined for 'Candidatus Phytoplasma rubi' confirmed the uniqueness of this phytoplasma strain from Portugal for which a novel ribosomal subgroup, 16SrV-I, is proposed. The representative of this new subgroup was named blackPort phytoplasma (Portuguese blackberry phytoplasma).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Systematic and Evolutionary Microbiology
ISSN
1466-5026
e-ISSN
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Volume of the periodical
66
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
1438-1446
UT code for WoS article
000374102200053
EID of the result in the Scopus database
2-s2.0-84962054050