CpSAT-1, a transcribed satellite sequence from the codling moth, Cydia pomonella

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00468806" target="_blank" >RIV/60077344:_____/16:00468806 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/16:43891089

Result on the web

<a href="http://dx.doi.org/10.1007/s10709-016-9907-0" target="_blank" >http://dx.doi.org/10.1007/s10709-016-9907-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10709-016-9907-0" target="_blank" >10.1007/s10709-016-9907-0</a>

Result language

angličtina

Original language name

CpSAT-1, a transcribed satellite sequence from the codling moth, Cydia pomonella

Original language description

Satellite DNA (satDNA) is a non-coding component of eukaryotic genomes, located mainly in heterochromatic regions. Relevance of satDNA began to emerge with accumulating evidence of its potential yet hardly comprehensible role that it can play in the genome of many organisms. We isolated the first satDNA of the codling moth (Cydia pomonella, Tortricidae, Lepidoptera), a species with holokinetic chromosomes and a single large heterochromatic element, the W chromosome in females. The satDNA, called CpSAT-1, is located on all chromosomes of the complement, although in different amounts. Surprisingly, the satellite is almost missing in the heterochromatic W chromosome. Additionally, we isolated mRNA from all developmental stages (1st-5th instar larva, pupa, adult), both sexes (adult male and female) and several tissues (Malpighian tubules, gut, heart, testes, and ovaries) of the codling moth and showed the CpSAT-1 sequence was transcribed in all tested samples. Using CpSAT-1 specific primers we amplified, cloned and sequenced 40 monomers from cDNA and gDNA, respectively. The sequence analysis revealed a high mutation rate and the presence of potentially functional motifs, mainly in non-conserved regions of the monomers. Both the chromosomal distribution and the sequence analysis suggest that CPSAT-1 has no function in the C. pomonella genome.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genetica

ISSN

0016-6707

e-ISSN

—

Volume of the periodical

144

Issue of the periodical within the volume

4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

385-395

UT code for WoS article

000384909200002

EID of the result in the Scopus database

2-s2.0-84970998116