Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00472830" target="_blank" >RIV/60077344:_____/16:00472830 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/16:43890539 RIV/60076658:12520/16:43890539
Result on the web
<a href="http://dx.doi.org/10.1002/mrd.22737" target="_blank" >http://dx.doi.org/10.1002/mrd.22737</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mrd.22737" target="_blank" >10.1002/mrd.22737</a>
Alternative languages
Result language
angličtina
Original language name
Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)
Original language description
The purpose of the study was to analyze seminal plasma composition, sperm production, and sperm motility over the course of the spawning season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss). The highest mean percent of motile sperm (carp, 98.3 +/- 1.6%; rainbow trout, 92.8 +/- 5.6%) and highest spermatozoon velocity (carp, 286.3 +/- 7.8msec(-1); rainbow trout, 245.3 +/- 8.3msec(-1)) were observed in the middle phase of the spawning period, at 5sec post-activation. Sperm volume and concentration increased in the early spawning period, then decreased significantly toward the end of the season in both species. Seminal plasma osmolality was 262-270mOsmolkg(-1) from carp and 232-248mOsmolkg(-1) from rainbow trout, with little variation over the spawning period. Protein concentration in carp seminal plasma was stable throughout the reproductive season, whereas the highest protein level (2.1 +/- 0.3mgmL(-1)) in rainbow trout was observed in the middle phase of the spawning period. Seminal plasma composition in both species was analyzed using two-dimensional polyacrylamide gel electrophoresis combined with mass spectrometry. Thirteen differentially expressed protein spots in carp seminal plasma and sixteen spots in rainbow trout seminal plasma varied over the course of the reproductive season. The unique proteins identified are involved in the regulation of spermatogenesis, sperm motility, maintenance of sperm membrane lipid stability, and antioxidant protection. These results provide a deeper understanding of the role of fish seminal plasma proteins as well as a list of novel markers of sperm quality. Mol. Reprod. Dev. 83: 968-982, 2016 (c) 2016 Wiley Periodicals, Inc.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Reproduction and Development
ISSN
1040-452X
e-ISSN
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Volume of the periodical
83
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
968-982
UT code for WoS article
000387859100004
EID of the result in the Scopus database
2-s2.0-84995389660