Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00475314" target="_blank" >RIV/60077344:_____/17:00475314 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/17:00475314 RIV/60076658:12310/17:43895799

Result on the web

<a href="http://dx.doi.org/10.1111/1744-7917.12314" target="_blank" >http://dx.doi.org/10.1111/1744-7917.12314</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1744-7917.12314" target="_blank" >10.1111/1744-7917.12314</a>

Result language

angličtina

Original language name

Adipokinetic hormones control amylase activity in the cockroach (Periplaneta americana) gut

Original language description

This study examined the biochemical characteristics of alpha-amylase and hormonal (adipokinetic hormone: AKH) stimulation of alpha-amylase activity in the cockroach (Periplaneta americana) midgut. We applied two AKHs in vivo and in vitro, then measured resultant amylase activity and gene expression, as well as the expression of AKH receptor (AKHR). The results revealed that optimal amylase activity is characterized by the following: pH: 5.7, temperature: 38.4 degrees C, K-m (Michaelis-Menten constant): 2.54 mg starch/mL, and V-max (maximum reaction velocity): 0.185 mu mol maltose/mL/min. In vivo application of AKHs resulted in significant increase of amylase activity: by two-fold in the gastric caeca and 4-7 fold in the rest of the midgut. In vitro experiments supported results seen in vivo: a 24-h incubation with the hormones resulted in the increase of amylase activity by 1.4 times in the caeca and 4-9 times in the midgut. Further, gene expression analyses reveal that AKHR is expressed in both the caeca and the rest of the midgut, although expression levels in the former were 23 times higher than levels in the latter. A similar pattern was found for the amylase (AMY) gene. Hormonal treatment did not affect the expression of either gene. This study is the first to provide evidence indicating direct AKH stimulation of digestive enzyme activity in the insect midgut, supported by specific AKHR gene expression in this organ.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA14-07172S" target="_blank" >GA14-07172S: Anti-stress reactions regulating metabolic homeostasis in insects</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Insect Science

ISSN

1672-9609

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

259-269

UT code for WoS article

000398603200008

EID of the result in the Scopus database

2-s2.0-84962641165