Analysis of tick-borne encephalitis virus-induced host responses in human cells of neuronal origin and interferon-mediated protection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00479247" target="_blank" >RIV/60077344:_____/17:00479247 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/17:43895738

Result on the web

<a href="http://dx.doi.org/10.1099/jgv.0.000853" target="_blank" >http://dx.doi.org/10.1099/jgv.0.000853</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/jgv.0.000853" target="_blank" >10.1099/jgv.0.000853</a>

Result language

angličtina

Original language name

Analysis of tick-borne encephalitis virus-induced host responses in human cells of neuronal origin and interferon-mediated protection

Original language description

Tick-borne encephalitis virus (TBEV) is a member of the genus Flavivirus. It can cause serious infections in humans that may result in encephalitis/meningoencephalitis. Although several studies have described the involvement of specific genes in the host response to TBEV infection in the central nervous system (CNS), the overall network remains poorly characterized. Therefore, we investigated the response of DAOY cells (human medulloblastoma cells derived from cerebellar neurons) to TBEV (Neudoerfl strain, Western subtype) infection to characterize differentially expressed genes by transcriptome analysis. Our results revealed a wide panel of interferon-stimulated genes (ISGs) and pro-inflammatory cytokines, including type III but not type I (or II) interferons (IFNs), which are activated upon TBEV infection, as well as a number of non-coding RNAs, including long non-coding RNAs. To obtain a broader view of the pathways responsible for eliciting an antiviral state in DAOY cells we examined the effect of type I and III IFNs and found that only type I IFN pretreatment inhibited TBEV production. The cellular response to TBEV showed only partial overlap with gene expression changes induced by IFN-beta treatment suggesting a virus-specific signature and we identified a group of ISGs that were highly up-regulated following IFN-beta treatment. Moreover, a high rate of down-regulation was observed for a wide panel of pro-inflammatory cytokines upon IFN-beta treatment. These data can serve as the basis for further studies of host-TBEV interactions and the identification of ISGs and/or lncRNAs with potent antiviral effects in cases of TBEV infection in human neuronal cells.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of General Virology

ISSN

0022-1317

e-ISSN

—

Volume of the periodical

98

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

2043-2060

UT code for WoS article

000410479400009

EID of the result in the Scopus database

2-s2.0-85028814431