Polyhydroxybutyrate production by an extremely halotolerant Halomonas elongata strain isolated from the hypersaline meromictic Fr Fund Lake (Transylvanian Basin, Romania)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F18%3A00495368" target="_blank" >RIV/60077344:_____/18:00495368 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/jam.14029" target="_blank" >http://dx.doi.org/10.1111/jam.14029</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jam.14029" target="_blank" >10.1111/jam.14029</a>

Result language

angličtina

Original language name

Polyhydroxybutyrate production by an extremely halotolerant Halomonas elongata strain isolated from the hypersaline meromictic Fr Fund Lake (Transylvanian Basin, Romania)

Original language description

Aim: This study aimed at unprecedented physical and chemical evaluation of the green plastics' polyhydroxyalkanoates (PHAs), in an extremely halotolerant Halomonas elongata strain 2FF under high-salt concentration.nMethods and Results: ConclusionsThe investigated bacterial strain was isolated from the surface water of the hypersaline Fr Fund Lake. The 16S rRNA gene sequence phylogeny and phenotypic analysis indicated that the isolate belonged to H. elongata. PHA inclusions were observed by Sudan Black B, Nile Red staining, and transmission electron microscopy during growth at high salinity (10%, w/v, NaCl) on 1% (w/v) d-glucose. The produced polymer was quantitatively and qualitatively assessed using crotonic acid assay, elemental analysis, Fourier transform infrared and Raman spectroscopies. Additionally, X-ray powder diffraction, H-1-NMR spectroscopy, and differential scanning calorimetry were applied. The investigations showed that the intracellular polymer was polyhydroxybutyrate (PHB) of which the strain produced up to 40 wt% of total cell dry weight after 48h. The analysis of phaC gene from the isolated H. elongata strain indicated that the encoded PHA synthase belongs to Class I PHA synthase family. Conclusions: Overall, our investigations pointed out that the halotolerant H.elongata strain 2FF was capable to produce significant amounts of PHB fromd-glucose, and PHAs from various carbon substrates at high-salt concentrations. Significance and Impact of the Study: The tested strain showed the ability for significant production of natural, biodegradable polymers under nutrient limitation and hypersaline conditions suggesting its potentiality for further metabolic and molecular investigations towards enhanced biopolymer production. Additionally, this study reports on the unprecedented use of Raman and XPRD techniques to investigate PHAs of an extremely halotolerant bacterium, thus expanding the repertoire of physical methods to study green plastics derived from extremophilic microorganisms.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

—

Volume of the periodical

125

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

1343-1357

UT code for WoS article

000447408400011

EID of the result in the Scopus database

2-s2.0-85052661087