The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00496711" target="_blank" >RIV/60077344:_____/19:00496711 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/19:00496711 RIV/60076658:12310/19:43899328
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0965174818303837?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0965174818303837?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ibmb.2018.11.003" target="_blank" >10.1016/j.ibmb.2018.11.003</a>
Alternative languages
Result language
angličtina
Original language name
The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella
Original language description
Lepidopteran silk is a complex assembly of proteins produced by a pair of highly specialized labial glands called silk glands. Silk composition has been examined only in a handful of species. Here we report on the analysis of silk gland-specific transcriptomes from three developmental stages of the greater wax moth, Galleria mellonella, combined with proteomics, Edman microsequencing and northern blot analysis. In addition to the genes known earlier, we identified twenty seven candidate cDNAs predicted to encode secretory proteins, which may represent novel silk components. Eight were verified by proteomic analysis or microsequencing, and several others were confirmed by similarity with known silk genes and their expression patterns. Our results revealed that most candidates encode abundant secreted proteins produced by middle silk glands including ten sericins, two seroins, one or more mucins, and several sequences share no apparent similarity to known proteins. Our data not only show that the number of sericin genes in the greater wax moth is higher than in other species thus far examined, but also the total content of soluble proteins in silk is twice as high in G. mellonella as in B. mori or A. yamamai. Our data will serve as a foundation for future identification and evolutionary analysis of silk proteins in the Lepidoptera.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LTC17073" target="_blank" >LTC17073: Silk sericins as biodegradable natural adhesives</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Insect Biochemistry and Molecular Biology
ISSN
0965-1748
e-ISSN
—
Volume of the periodical
106
Issue of the periodical within the volume
MAR 01
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
28-38
UT code for WoS article
000468712100004
EID of the result in the Scopus database
2-s2.0-85057759359