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The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00496711" target="_blank" >RIV/60077344:_____/19:00496711 - isvavai.cz</a>

  • Alternative codes found

    RIV/68378050:_____/19:00496711 RIV/60076658:12310/19:43899328

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0965174818303837?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0965174818303837?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ibmb.2018.11.003" target="_blank" >10.1016/j.ibmb.2018.11.003</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The expansion of genes encoding soluble silk components in the greater wax moth, Galleria mellonella

  • Original language description

    Lepidopteran silk is a complex assembly of proteins produced by a pair of highly specialized labial glands called silk glands. Silk composition has been examined only in a handful of species. Here we report on the analysis of silk gland-specific transcriptomes from three developmental stages of the greater wax moth, Galleria mellonella, combined with proteomics, Edman microsequencing and northern blot analysis. In addition to the genes known earlier, we identified twenty seven candidate cDNAs predicted to encode secretory proteins, which may represent novel silk components. Eight were verified by proteomic analysis or microsequencing, and several others were confirmed by similarity with known silk genes and their expression patterns. Our results revealed that most candidates encode abundant secreted proteins produced by middle silk glands including ten sericins, two seroins, one or more mucins, and several sequences share no apparent similarity to known proteins. Our data not only show that the number of sericin genes in the greater wax moth is higher than in other species thus far examined, but also the total content of soluble proteins in silk is twice as high in G. mellonella as in B. mori or A. yamamai. Our data will serve as a foundation for future identification and evolutionary analysis of silk proteins in the Lepidoptera.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LTC17073" target="_blank" >LTC17073: Silk sericins as biodegradable natural adhesives</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Insect Biochemistry and Molecular Biology

  • ISSN

    0965-1748

  • e-ISSN

  • Volume of the periodical

    106

  • Issue of the periodical within the volume

    MAR 01

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

    28-38

  • UT code for WoS article

    000468712100004

  • EID of the result in the Scopus database

    2-s2.0-85057759359