Increased methane concentration alters soil prokaryotic community structure along an artificial pH gradient

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00505532" target="_blank" >RIV/60077344:_____/19:00505532 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/19:10403132

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs13213-018-1421-4" target="_blank" >https://link.springer.com/article/10.1007%2Fs13213-018-1421-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13213-018-1421-4" target="_blank" >10.1007/s13213-018-1421-4</a>

Result language

angličtina

Original language name

Increased methane concentration alters soil prokaryotic community structure along an artificial pH gradient

Original language description

Global climate change may have a large impact on increased emission rates of carbon dioxide and methane to total greenhouse gas emissions from terrestrial wetlands. Methane consumption by soil microbiota in alpine wet meadows serves as a biofilter for the methane produced in the waterlogged soil below. Altered pH regimes change microbial community composition and structure by exerting selection pressure on soil microorganisms with different ecological strategies and thus affect greenhouse gas emissions resulting from the metabolic activity of soil microorganisms. However, responses of prokaryotic communities to artificial pH shift under elevated methane concentration remain unclear. In this study, we assessed diversity and relative abundance of soil prokaryotes in an alpine meadow under elevated methane concentration along an artificial pH gradient using laboratory incubation experiments. We established an incubation experiment treated with artificial pH gradient (pH 4.5-8.5). After 3 months of incubation, 300 ml of methane at a concentration of 20,000 ppm was added to stimulate potential methanothrophs in topsoil. Sequencing of 16S rRNA gene indicated increasing of relative abundances of Crenarchaeota, Chloroflexi, Bacteroidetes, and Planctomycetes in soil after addition of methane, while the relative abundances of Actinobacteria and Gemmatimonadetes did not significant change before and after methane treatment. Results of phylogenetic relatedness of soil prokaryotes showed that microbial community is mostly shaped by deterministic factors. Species indicator analysis revealed distinct OTUs among various pH and methane treatments. Network analysis revealed distinct co-occurrence patterns of soil prokaryotic community before and after methane addition, and different correlation patterns among various prokaryotic taxa. Linear regression model revealed significant decrease of methane oxidation along elevated pH gradient. Soil pH constituted a strong environmental filter in species assembly of soil prokaryotic community. Methane oxidation rates decreased significantly with elevated pH. The interactive effects of elevated methane concentration and pH are therefore promising topic for future research.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Microbiology

ISSN

1590-4261

e-ISSN

—

Volume of the periodical

69

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

329-339

UT code for WoS article

000462829700003

EID of the result in the Scopus database

2-s2.0-85059683613