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New Halonotius Species Provide Genomics-Based Insights Into Cobalamin Synthesis in Haloarchaea

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00509914" target="_blank" >RIV/60077344:_____/19:00509914 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.frontiersin.org/articles/10.3389/fmicb.2019.01928/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2019.01928/full</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fmicb.2019.01928" target="_blank" >10.3389/fmicb.2019.01928</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    New Halonotius Species Provide Genomics-Based Insights Into Cobalamin Synthesis in Haloarchaea

  • Original language description

    Hypersaline aquatic and terrestrial ecosystems display a cosmopolitan distribution. These environments teem with microbes and harbor a plethora of prokaryotic lineages that evaded ecological characterization due to the prior inability to cultivate them or to access their genomic information. In order to close the current knowledge gap, we performed two sampling and isolation campaigns in the saline soils of the Odiel Saltmarshes and the salterns of Isla Cristina (Huelva, Spain). From the isolated haloarchaeal strains subjected to high-throughput phylogenetic screening, two were chosen (F15B(T) and F9-27(T)) for physiological and genomic characterization due of their relatedness to the genus Halonotius. Comparative genomic analyses were carried out between the isolated strains and the genomes of previously described species Halonotius pteroides CECT 7525(T), Halonotius aquaticus F13-13(T) and environmentaly recovered metagenome-assembled representatives of the genus Halonotius. The topology of the phylogenomic tree showed agreement with the phylogenetic ones based on 16S rRNA and rpoB' genes, and together with average amino acid and nucleotide identities suggested the two strains as novel species within the genus. We propose the names Halonotius terrestris sp. nov. (type strain F15B(T) = CECT 9688(T) = CCM 8954(T)) and Halonotius roseus sp. nov. (type strain F9-27(T) = CECT 9745(T) = CCM 8956(T)) for these strains. Comparative genomic analyses within the genus highlighted a typical salt-in signature, characterized by acidic proteomes with low isoelectric points, and indicated heterotrophic aerobic lifestyles. Genome-scale metabolic reconstructions revealed that the newly proposed species encode all the necessary enzymatic reactions involved in cobalamin (vitamin B-12) biosynthesis. Based on the worldwide distribution of the genus and its abundance in hypersaline habitats we postulate that its members perform a critical function by being able to provide expensive commodities (i.e., vitamin B-12) to the halophilic microbial communities at large.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/GA17-04828S" target="_blank" >GA17-04828S: Unveiling life strategies of uncultivated viruses in freshwater environments using metagenomics</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Microbiology

  • ISSN

    1664-302X

  • e-ISSN

  • Volume of the periodical

    10

  • Issue of the periodical within the volume

    AUG

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    19

  • Pages from-to

    1928

  • UT code for WoS article

    000482803800001

  • EID of the result in the Scopus database

    2-s2.0-85071943645