Stable isotope probing techniques and methodological considerations using 15N

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00510257" target="_blank" >RIV/60077344:_____/19:00510257 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-9721-3_14" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-9721-3_14</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-9721-3_14" target="_blank" >10.1007/978-1-4939-9721-3_14</a>

Result language
angličtina
Original language name
Stable isotope probing techniques and methodological considerations using 15N
Original language description
Nitrogen fixation and assimilation processes are vital to the functioning of any ecosystem. Nevertheless, studying these processes using 15N-based stable isotope probing was so far limited because of technical challenges related to the relative rarity of nitrogen in nucleic acids and proteins compared to carbon, and because of its absence in lipids. However, the recent adoption of high-throughput sequencing and statistical modelling methods to SIP studies increased the sensitivity of the method and enabled overcoming some of the challenges. This chapter describes in detail how to perform DNA- and RNA-SIP using 15N.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
Stable Isotope Probing: Methods and Protocols, Methods in Molecular Biology
ISBN
978-1-4939-9720-6
Number of pages of the result
13
Pages from-to
175-187
Number of pages of the book
247
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
000517291000015

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