Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00511209" target="_blank" >RIV/60077344:_____/19:00511209 - isvavai.cz</a>
Result on the web
<a href="https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13065" target="_blank" >https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13065</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppa.13065" target="_blank" >10.1111/ppa.13065</a>

Result language
angličtina
Original language name
Molecular and biological properties of two putative new cytorhabdoviruses infecting Trifolium pratense
Original language description
Three pairs of generic primers designed for specific reverse transcription polymerase chain reaction amplification of part of the cytorhabdoviruses genomes were successfully tested with a number of red clover samples. Two infected plants showing irregular vein clearing, tissue necrosis and stunted growth symptoms were selected for further studies. Subsequently, two novel plant rhabdoviruses, tentatively named Trifolium pratense virus A (TpVA) and Trifolium pratense virus B (TpVB), were detected in red clover plants. Sequence analyses of nucleotide sequences of their genomic RNAs obtained after high-throughput sequencing confirmed their affinity to members of the genus Cytorhabdovirus. While TpVA was transmitted by mechanical inoculation to Nicotiana occidentalis 37B and subtransferred to Physalis floridana plants, crude sap transmission of TpVB to a range of herbaceous host plants failed. The bacilliform shape of virions, which has a cytoplasm-limited distribution, their sizes, and the phylogenetic relationships, support assignment of TpVA and TpVB to two distinct species of the genus Cytorhabdovirus.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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