Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00532452" target="_blank" >RIV/60077344:_____/20:00532452 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/21/10/3488/htm" target="_blank" >https://www.mdpi.com/1422-0067/21/10/3488/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21103488" target="_blank" >10.3390/ijms21103488</a>

Result language
angličtina
Original language name
Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture
Original language description
Centromeres are essential for proper chromosome segregation to the daughter cells during mitosis and meiosis. Chromosomes of most eukaryotes studied so far have regional centromeres that form primary constrictions on metaphase chromosomes. These monocentric chromosomes vary from point centromeres to so-called ´meta-polycentromeres´, with multiple centromere domains in an extended primary constriction, as identified in Pisum and Lathyrus species. However, in various animal and plant lineages centromeres are distributed along almost the entire chromosome length. Therefore, they are called holocentromeres. In holocentric plants, centromere-specific proteins, at which spindle fibers usually attach, are arranged contiguously (line-like), in clusters along the chromosomes or in bands. Here, we summarize findings of ultrastructural investigations using immunolabeling with centromere-specific antibodies and super-resolution microscopy to demonstrate the structural diversity of plant centromeres. A classification of the different centromere types has been suggested based on the distribution of spindle attachment sites. Based on these findings we discuss the possible evolution and advantages of holocentricity, and potential strategies to segregate holocentric chromosomes correctly.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)

Project
<a href="/en/project/GA17-09750S" target="_blank" >GA17-09750S: Utilizing new models and technologies to elucidate centromere determination in plants</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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