Simultaneous presence of bacteriochlorophyll and xanthorhodopsin genes in a freshwater bacterium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00536958" target="_blank" >RIV/60077344:_____/20:00536958 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/20:00536958

Result on the web

<a href="https://msystems.asm.org/content/5/6/e01044-20" target="_blank" >https://msystems.asm.org/content/5/6/e01044-20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/mSystems.01044-20" target="_blank" >10.1128/mSystems.01044-20</a>

Result language

angličtina

Original language name

Simultaneous presence of bacteriochlorophyll and xanthorhodopsin genes in a freshwater bacterium

Original language description

Photoheterotrophic bacteria represent an important part of aquatic microbial communities. There exist two fundamentally different light-harvesting systems: bacteriochlorophyll-containing reaction centers or rhodopsins. Here, we report a photoheterotrophic Sphingomonas strain isolated from an oligotrophic lake, which contains complete sets of genes for both rhodopsin-based and bacteriochlorophyllbased phototrophy. Interestingly, the identified genes were not expressed when cultured in liquid organic media. Using reverse transcription quantitative PCR (RT-qPCR), RNA sequencing, and bacteriochlorophyll a quantification, we document that bacteriochlorophyll synthesis was repressed by high concentrations of glucose or galactose in the medium. Coactivation of photosynthesis genes together with genes for TonBdependent transporters suggests the utilization of light energy for nutrient import. The photosynthetic units were formed by ring-shaped light-harvesting complex 1 and reaction centers with bacteriochlorophyll a and spirilloxanthin as the main lightharvesting pigments. The identified rhodopsin gene belonged to the xanthorhodopsin family, but it lacks salinixanthin antenna. In contrast to bacteriochlorophyll, the expression of xanthorhodopsin remained minimal under all experimental conditions tested. Since the gene was found in the same operon as a histidine kinase, we propose that it might serve as a light sensor. Our results document that photoheterotrophic Sphingomonas bacteria use the energy of light under carbon-limited conditions, while under carbon-replete conditions, they cover all their metabolic needs through oxidative phosphorylation. IMPORTANCE Phototrophic organisms are key components of many natural environments. There exist two main phototrophic groups: species that collect light energy using various kinds of (bacterio)chlorophylls and species that utilize rhodopsins

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GJ18-14095Y" target="_blank" >GJ18-14095Y: Aerobic anoxygenic phototrophs: their role in carbon utilization in light in freshwater ecosystems (AAPs rule!)</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

mSystems

ISSN

2379-5077

e-ISSN

2379-5077

Volume of the periodical

5

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

e01044-20

UT code for WoS article

000630974900017

EID of the result in the Scopus database

2-s2.0-85098211480