Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00540542" target="_blank" >RIV/60077344:_____/20:00540542 - isvavai.cz</a>

Result on the web

<a href="https://jcs.biologists.org/content/133/3/jcs238659" target="_blank" >https://jcs.biologists.org/content/133/3/jcs238659</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.238659" target="_blank" >10.1242/jcs.238659</a>

Result language

angličtina

Original language name

Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila

Original language description

In endolysosomal networks, two hetero-hexameric tethers called HOPS and CORVET are found widely throughout eukaryotes. The unicellular ciliate Tetrahymena thermophila possesses elaborate endolysosomal structures, but curiously both it and related protozoa lack the HOPS tether and several other trafficking proteins, while retaining the related CORVET complex. Here, we show that Tetrahymena encodes multiple paralogs of most CORVET subunits, which assemble into six distinct complexes. Each complex has a unique subunit composition and, significantly, shows unique localization, indicating participation in distinct pathways. One pair of complexes differ by a single subunit (Vps8), but have late endosomal versus recycling endosome locations. While Vps8 subunits are thus prime determinants for targeting and functional specificity, determinants exist on all subunits except Vps11. This unprecedented expansion and diversification of CORVET provides a potent example of tether flexibility, and illustrates how 'backfilling' following secondary losses of trafficking genes can provide a mechanism for evolution of new pathways.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

1477-9137

e-ISSN

—

Volume of the periodical

133

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

jcs238659

UT code for WoS article

000518434400015

EID of the result in the Scopus database

2-s2.0-85079352630