Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00554280" target="_blank" >RIV/60077344:_____/21:00554280 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/21:10432880 RIV/60460709:41210/21:88263 RIV/62157124:16170/21:43879116 RIV/62157124:16810/21:43879116 and 2 more
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/zph.12883" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/zph.12883</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/zph.12883" target="_blank" >10.1111/zph.12883</a>
Alternative languages
Result language
angličtina
Original language name
Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods
Original language description
European brown hare (Lepus europaeus Pallas 1778) is a broadly distributed lagomorph species in Europe, recognized as a host for Ixodes ricinus and reservoir of a wide range of pathogens with zoonotic potential. Even though Lepus europaeus represents an important game animal in Central Europe, the data available on Anaplasma phagocytophilum in this lagomorph are scarce. In this study, three populations of brown hare from distinct localities in the Czech Republic were analysed for the presence of Anaplasma phagocytophilum DNA. We used standard qPCR, targeting the msp2 gene and adapted the same assay also for digital droplet PCR. Out of 91 samples, these two methods identified 9 and 12 as positive, respectively. For taxonomic analysis, we amplified the groEL gene from five of six samples that were found positive by both methods. In phylogenetic analyses, this haplotype belongs to ecotype 1, and to the subclade with isolates from cervids and I. ricinus. Our findings underline the importance of correct result interpretation and positivity cut-off set-up for different detection methods of A. phagocytophilum. This bacterium is characterized by a high intraspecific variability and highly sensitive detection itself, is not enough. Detailed molecular typing is necessary to define the zoonotic potential of different strains and their natural reservoirs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Zoonoses and Public Health
ISSN
1863-1959
e-ISSN
1863-2378
Volume of the periodical
68
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
917-925
UT code for WoS article
000683888600001
EID of the result in the Scopus database
2-s2.0-85112245550