Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00555231" target="_blank" >RIV/60077344:_____/21:00555231 - isvavai.cz</a>
Result on the web
<a href="https://journals.asm.org/doi/10.1128/mBio.01288-21" target="_blank" >https://journals.asm.org/doi/10.1128/mBio.01288-21</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/mBio.01288-21" target="_blank" >10.1128/mBio.01288-21</a>
Alternative languages
Result language
angličtina
Original language name
Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
Original language description
Borrelia burgdorferi, the etiological agent of Lyme disease, persists in nature through an enzootic cycle consisting of a vertebrate host and an Ixodes tick vector. The sequence motifs modified by two well-characterized restriction/modification (R/M) loci of B. burgdorferi type strain B31 were recently described, but the methylation profiles of other Lyme disease Borrelia bacteria have not been characterized. Here, the methylomes of B. burgdorferi type strain B31 and 7 clonal derivatives, along with B. burgdorferi N40, B. burgdorferi 297, B. burgdorferi CA-11, B. afzelii PKo, B. afzelii BO23, and B. garinii PBr, were defined through PacBio single-molecule realtime (SMRT) sequencing. This analysis revealed 9 novel sequence motifs methylated by the plasmid-encoded restriction/modification enzymes of these Borrelia strains. Furthermore, while a previous analysis of B. burgdorferi B31 revealed an epigenetic impact of methylation on the global transcriptome, the current data contradict those findings, our analyses of wild-type B. burgdorferi B31 revealed no consistent differences in gene expression among isogenic derivatives lacking one or more restriction/ modification enzymes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
mBio
ISSN
2161-2129
e-ISSN
2150-7511
Volume of the periodical
12
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
e01288-21
UT code for WoS article
000693451600003
EID of the result in the Scopus database
2-s2.0-85112119847