A New Model Trypanosomatid, Novymonas esmeraldas: Genomic Perception of Its Candidatus Pandoraea novymonadis Endosymbiont

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00555255" target="_blank" >RIV/60077344:_____/21:00555255 - isvavai.cz</a>

Result on the web

<a href="https://journals.asm.org/doi/10.1128/mBio.01606-21" target="_blank" >https://journals.asm.org/doi/10.1128/mBio.01606-21</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/mBio.01606-21" target="_blank" >10.1128/mBio.01606-21</a>

Result language

angličtina

Original language name

A New Model Trypanosomatid, Novymonas esmeraldas: Genomic Perception of Its Candidatus Pandoraea novymonadis Endosymbiont

Original language description

The closest relative of human pathogen Leishmania, the trypanosomatid Novymonas esmeraldas, harbors a bacterial endosymbiont Candidatus Pandoraea novymonadis. Based on genomic data, we performed a detailed characterization of the metabolic interactions of both partners. While in many respects the metabolism of N. esmeraldas resembles that of other Leishmaniinae, the endosymbiont provides the trypanosomatid with heme, essential amino acids, purines, some coenzymes, and vitamins. In return, N. esmeraldas shares with the bacterium several nonessential amino acids and phospholipids. Moreover, it complements its carbohydrate metabolism and urea cycle with enzymes missing from the Ca. Pandoraea novymonadis genome. The removal of the endosymbiont from N. esmeraldas results in a significant reduction of the overall translation rate, reduced expression of genes involved in lipid metabolism and mitochondrial respiratory activity, and downregulation of several aminoacyl-tRNA synthetases, enzymes involved in the synthesis of some amino acids, as well as proteins associated with autophagy. At the same time, the genes responsible for protection against reactive oxygen species and DNA repair become signifi- cantly upregulated in the aposymbiotic strain of this trypanosomatid. By knocking out a component of its flagellum, we turned N. esmeraldas into a new model trypanosomatid that is amenable to genetic manipulation using both conventional and CRISPR-Cas9-mediated approaches.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

mBio

ISSN

2161-2129

e-ISSN

2150-7511

Volume of the periodical

12

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

18

Pages from-to

e01606-21

UT code for WoS article

000696627000007

EID of the result in the Scopus database

2-s2.0-85114296400