Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00556708" target="_blank" >RIV/60077344:_____/22:00556708 - isvavai.cz</a>

Alternative codes found

RIV/68081766:_____/22:00556708 RIV/00216224:14310/22:00128113 RIV/60460709:41210/22:92359 RIV/62157124:16170/22:43879899

Result on the web

<a href="https://folia.paru.cas.cz/pdfs/fol/2022/01/07.pdf" target="_blank" >https://folia.paru.cas.cz/pdfs/fol/2022/01/07.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14411/fp.2022.007" target="_blank" >10.14411/fp.2022.007</a>

Result language

angličtina

Original language name

Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs

Original language description

Based on previously published data, the Czech Republic is regarded an endemic country of the onchocercid nematodes Dirofilaria immitis (Leidy, 1856) and Dirofilaria repens Railliet et Henry, 1911. Nevertheless, while cases of D. repens are commonly reported from dogs in South Moravia, no recent records of D. immitis are available. Therefore, the present study was performed to clarify the occurrence of both species ofDirofilaria Railliet et Henry, 1910. Blood samples of 551 dogs sampled during 2015 and 2016 were analysed microscopically for presence of microfilariae and blood sera were examined by IDEXX SNAP (R) 4Dx (R) test (IDEXX, USA). DNA from blood of microscopically positive dogs was extracted and PCR protocol amplifying fragment of cytochrome c oxi-dase I (COI) gene was performed, PCR products were then sequenced. All dogs from the Bohemian part of the Czech Republic were negative. The prevalence of D. repens in the Moravian region was 5.7% (27/476). BLAST analyses of obtained sequences confirmed the presence of D. repens (99-100% identical to KX265049). All sampled animals showed a negative result for D. immitis antigen in IDEXX SNAP (R) 4Dx (R) test. Our study confirmed the previously reported occurrence of D. repens in South Moravia and revealed its spreading from the epicentre to the north and west. PCR with subsequent sequencing together with negative results for D. immitis antigen in IDEXX SNAP (R) 4Dx (R) test revealed only D. repens infection. A previously published autochthonous infection of dogs with D. immitis in South Moravia was not confirmed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Parasitologica

ISSN

0015-5683

e-ISSN

1803-6465

Volume of the periodical

69

Issue of the periodical within the volume

007

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

007

UT code for WoS article

000779883000001

EID of the result in the Scopus database

2-s2.0-85128876494