Estimating biomass and vitality of microalgae for monitoring cultures: A roadmap for reliable measurements.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00560257" target="_blank" >RIV/60077344:_____/22:00560257 - isvavai.cz</a>
Alternative codes found
RIV/00216275:25310/22:39919260
Result on the web
<a href="https://doi.org/10.3390/cells11152455" target="_blank" >https://doi.org/10.3390/cells11152455</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cells11152455" target="_blank" >10.3390/cells11152455</a>
Alternative languages
Result language
angličtina
Original language name
Estimating biomass and vitality of microalgae for monitoring cultures: A roadmap for reliable measurements.
Original language description
Estimating algal biomass is a prerequisite for monitoring growth of microalgae. Especially for large-scale production sites, the measurements must be robust, reliable, fast and easy to obtain. We compare the relevant parameters, discuss potential hurdles and provide recommendations to tackle these issues. The focus is on optical density and in vivo autofluorescence of chlorophyll, which have proven to be ideal candidates for monitoring purposes. Beyond biomass, cell vitality is also crucial for maintaining cultures. While maximizing biomass yield is often the primary consideration, some applications require adverse growth conditions for the synthesis of high-quality compounds. The non-invasive technique of pulse-amplified modulated (PAM) fluorescence measurements provides an ideal tool and is increasingly being employed due to ever more affordable devices. We compared three devices and studied the robustness of the dark fluorescence yield of photosystem II (Fv/Fm) at various cell densities. Although the so-called inner filter effects influence the fluorescence signal, the resulting Fv/Fm remain stable and robust over a wide range of cell densities due to mutual effects.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cells
ISSN
2073-4409
e-ISSN
2073-4409
Volume of the periodical
11
Issue of the periodical within the volume
15
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
2455
UT code for WoS article
000838935200001
EID of the result in the Scopus database
2-s2.0-85136343263