Untargeted Multimodal Metabolomics Investigation of the Haemonchus contortus Exsheathment Secretome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00560730" target="_blank" >RIV/60077344:_____/22:00560730 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2073-4409/11/16/2525" target="_blank" >https://www.mdpi.com/2073-4409/11/16/2525</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cells11162525" target="_blank" >10.3390/cells11162525</a>
Alternative languages
Result language
angličtina
Original language name
Untargeted Multimodal Metabolomics Investigation of the Haemonchus contortus Exsheathment Secretome
Original language description
In nematodes that invade the gastro-intestinal tract of the ruminant, the process of larval exsheathment marks the transition from the free-living to the parasitic stages of these parasites. To investigate the secretome associated with larval exsheathment, a closed in vitro system that effectively reproduces the two basic components of an anaerobic rumen environment (CO2 and 39 degrees C) was developed to trigger exsheathment in one of the most pathogenic and model gastrointestinal parasitic nematodes, Haemonchus contortus (barber's pole worm). This study reports the use of multimodal untargeted metabolomics and lipidomics methodologies to identify the metabolic signatures and compounds secreted during in vitro larval exsheathment in the H. contortus infective third-stage larva (iL3). A combination of statistical and chemoinformatic analyses using three analytical platforms revealed a panel of metabolites detected post exsheathment and associated with amino acids, purines, as well as select organic compounds. The major lipid classes identified by the non-targeted lipidomics method applied were lysophosphatidylglycerols, diglycerides, fatty acyls, glycerophospholipids, and a triglyceride. The identified metabolites may serve as metabolic signatures to improve tractability of parasitic nematodes for characterizing small molecule host-parasite interactions related to pathogenesis, vaccine and drug design, as well as the discovery of metabolic biomarkers.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cells
ISSN
2073-4409
e-ISSN
2073-4409
Volume of the periodical
11
Issue of the periodical within the volume
16
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
2525
UT code for WoS article
000847033100001
EID of the result in the Scopus database
2-s2.0-85137125667