Toward global integration of biodiversity big data: a harmonized metabarcode data generation module for terrestrial arthropods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00561298" target="_blank" >RIV/60077344:_____/22:00561298 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/22:43904730

Result on the web

<a href="https://academic.oup.com/gigascience/article-pdf/doi/10.1093/gigascience/giac065/45025316/giac065.pdf" target="_blank" >https://academic.oup.com/gigascience/article-pdf/doi/10.1093/gigascience/giac065/45025316/giac065.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/gigascience/giac065" target="_blank" >10.1093/gigascience/giac065</a>

Result language

angličtina

Original language name

Toward global integration of biodiversity big data: a harmonized metabarcode data generation module for terrestrial arthropods

Original language description

Metazoan metabarcoding is emerging as an essential strategy for inventorying biodiversity, with diverse projects currently generating massive quantities of community-level data. The potential for integrating across such data sets offers new opportunities to better understand biodiversity and how it might respond to global change. However, large-scale syntheses may be compromised if metabarcoding workflows differ from each other. There are ongoing efforts to improve standardization for the reporting of inventory data. However, harmonization at the stage of generating metabarcode data has yet to be addressed. A modular framework for harmonized data generation offers a pathway to navigate the complex structure of terrestrial metazoan biodiversity. Here, through our collective expertise as practitioners, method developers, and researchers leading metabarcoding initiatives to inventory terrestrial biodiversity, we seek to initiate a harmonized framework for metabarcode data generation, with a terrestrial arthropod module. We develop an initial set of submodules covering the 5 main steps of metabarcode data generation: (i) sample acquisition, (ii) sample processing, (iii) DNA extraction, (iv) polymerase chain reaction amplification, library preparation, and sequencing, and (v) DNA sequence and metadata deposition, providing a backbone for a terrestrial arthropod module. To achieve this, we (i) identified key points for harmonization, (ii) reviewed the current state of the art, and (iii) distilled existing knowledge within submodules, thus promoting best practice by providing guidelines and recommendations to reduce the universe of methodological options. We advocate the adoption and further development of the terrestrial arthropod module. We further encourage the development of modules for other biodiversity fractions as an essential step toward large-scale biodiversity synthesis through harmonization.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10619 - Biodiversity conservation

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

GigaScience

ISSN

2047-217X

e-ISSN

2047-217X

Volume of the periodical

11

Issue of the periodical within the volume

JULY 19

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

giac065

UT code for WoS article

000846739000079

EID of the result in the Scopus database

2-s2.0-85134557257