Abolition of egg diapause by ablation of suboesophageal ganglion in parental females is compatible with genetic engineering methods

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00561680" target="_blank" >RIV/60077344:_____/22:00561680 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43905095
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0022191022000841?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022191022000841?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jinsphys.2022.104438" target="_blank" >10.1016/j.jinsphys.2022.104438</a>

Result language
angličtina
Original language name
Abolition of egg diapause by ablation of suboesophageal ganglion in parental females is compatible with genetic engineering methods
Original language description
Microinjection of genetic material into non-diapause eggs is required for genetic engineering of silkworms. Besides diapause could be useful for maintaining transgenic lines, a drawback of this technology is that most standard silkworm strains and experimental lines of interest produce diapausing eggs. Several approaches have been developed to abolish diapause but none are very efficient. Here, we investigated the ablation of the suboesophageal ganglion (SG) in female pupae, which is a source of the hormone required to trigger egg diapause, as a mean to abolish diapause. We showed that SG-ablation is a reliable method to produce nondiapause eggs. Additionally, the challenge associated with lower fecundity of females with SG ablation was resolved by injecting pilocarpine into the mated female. We also investigated the suitability of nondiapause eggs laid by SG-ablated females for transgenesis, targeted mutagenesis, and induction of parthenogenetic development. Our results demonstrated SG-ablation to be a useful and simple method for expanding the possibilities associated with genetic engineering in silkworms.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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