Expression profiling and cellular localization of myxozoan minicollagens during nematocyst formation and sporogenesis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00565966" target="_blank" >RIV/60077344:_____/22:00565966 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43905337
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0020751922001047?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0020751922001047?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijpara.2022.07.002" target="_blank" >10.1016/j.ijpara.2022.07.002</a>
Alternative languages
Result language
angličtina
Original language name
Expression profiling and cellular localization of myxozoan minicollagens during nematocyst formation and sporogenesis
Original language description
In free-living cnidarians, minicollagens are major structural components in the biogenesis of nemato-cysts. Recent sequence mining and proteomic analysis demonstrate that minicollagens are also expressed by myxozoans, a group of evolutionarily ancient cnidarian endoparasites. Nonetheless, the presence and abundance of nematocyst-associated genes/proteins in nematocyst morphogenesis have never been studied in Myxozoa. Here, we report the gene expression profiles of three myxozoan minicollagens, ncol-1, ncol-3, and the recently identified noncanonical ncol-5, during the intrapiscine development of Myxidium lieberkuehni, the myxozoan parasite of the northern pike, Esox lucius. Moreover, we localized the myxozoan-specific minicollagen Ncol-5 in the developing myxosporean stages by Western blotting, immunofluorescence, and immunogold electron microscopy. We found that expression of minicollagens was spatiotemporally restricted to developing nematocysts within the myxospores during sporogenesis. Intriguingly, Ncol-5 is localized in the walls of nematocysts and predominantly in nematocyst tubules. Overall, we demonstrate that despite being significantly reduced in morphology, myxozoans retain struc-tural components associated with nematocyst development in free-living cnidarians. Furthermore, our findings have practical implications for future functional and comparative studies as minicollagens are useful markers of the developmental phase of myxozoan parasites.(c) 2022 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal for Parasitology
ISSN
0020-7519
e-ISSN
1879-0135
Volume of the periodical
52
Issue of the periodical within the volume
10
Country of publishing house
AU - AUSTRALIA
Number of pages
9
Pages from-to
667-675
UT code for WoS article
000894203300004
EID of the result in the Scopus database
2-s2.0-85136599425