Primary donor triplet states of Photosystem I and II studied by Q-band pulse ENDOR spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00568733" target="_blank" >RIV/60077344:_____/22:00568733 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11120-022-00905-y" target="_blank" >https://link.springer.com/article/10.1007/s11120-022-00905-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11120-022-00905-y" target="_blank" >10.1007/s11120-022-00905-y</a>
Alternative languages
Result language
angličtina
Original language name
Primary donor triplet states of Photosystem I and II studied by Q-band pulse ENDOR spectroscopy
Original language description
The photoexcited triplet state of the 'primary donors' in the two photosystems of oxygenic photosynthesis has been investigated by means of electron-nuclear double resonance (ENDOR) at Q-band (34 GHz). The data obtained represent the first set of H-1 hyperfine coupling tensors of the (3)P700 triplet state in PSI and expand the existing data set for (3)P680. We achieved an extensive assignment of the observed electron-nuclear hyperfine coupling constants (hfcs) corresponding to the methine alpha-protons and the methyl group beta-protons of the chlorophyll (Chl) macrocycle. The data clearly confirm that in both photosystems the primary donor triplet is located on one specific monomeric Chl at cryogenic temperature. In comparison to previous transient ENDOR and pulse ENDOR experiments at standard X-band (9-10 GHz), the pulse Q-band ENDOR spectra demonstrate both improved signal-to-noise ratio and increased resolution. The observed ENDOR spectra for (3)P700 and (3)P680 differ in terms of the intensity loss of lines from specific methyl group protons, which is explained by hindered methyl group rotation produced by binding site effects. Contact analysis of the methyl groups in the PSI crystal structure in combination with the ENDOR analysis of (3)P700 suggests that the triplet is located on the Chl aMODIFIER LETTER PRIME (P-A) in PSI. The results also provide additional evidence for the localization of (3)P680 on the accessory Chl(D1) in PSII.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/EF18_053%2F0016982" target="_blank" >EF18_053/0016982: International mobilities for researchers and administrative employees of Biology Centre</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Photosynthesis Research
ISSN
0166-8595
e-ISSN
1573-5079
Volume of the periodical
152
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
22
Pages from-to
213-234
UT code for WoS article
000769264600001
EID of the result in the Scopus database
2-s2.0-85126294903