Millipede gut-derived microbes as a potential source of cellulolytic enzymes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00571439" target="_blank" >RIV/60077344:_____/23:00571439 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12220/23:43906324 RIV/60076658:12520/23:43906324
Result on the web
<a href="https://link.springer.com/article/10.1007/s11274-023-03620-5" target="_blank" >https://link.springer.com/article/10.1007/s11274-023-03620-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11274-023-03620-5" target="_blank" >10.1007/s11274-023-03620-5</a>
Alternative languages
Result language
angličtina
Original language name
Millipede gut-derived microbes as a potential source of cellulolytic enzymes
Original language description
Lignocellulose biomass has recently been considered a cost-effective and renewable energy source within circular economy management. Cellulases are important key enzymes for simple, fast, and clean biomass decomposition. The intestinal tract of millipedes is the environment which can provide promising microbial strains with cellulolytic potential. In the present study, we used the tropical millipede Telodeinopus aoutii as an experimental organism. Within a feeding test in which millipedes were fed with oak and maple leaf litter, we focused on isolating culturable cellulolytic microbiota from the millipede gut. Several growth media selecting for actinobacteria, bacteria, and fungi have been used to cultivate microbial strains with cellulolytic activities. Our results showed that oak-fed millipedes provided a higher number of culturable bacteria and a more diversified microbial community than maple-fed ones. The screening for cellulolytic activity using Congo red revealed that about 30% of bacterial and fungal phylotypes isolated from the gut content of T. aoutii, produced active cellulases in vitro. Actinobacteria Streptomyces and Kitasatospora were the most active cellulolytic genera on Congo red test. In contrast, fungi Aspergillus, Penicillium, Cheatomium, Clonostachys, and Trichoderma showed the highest protein-specific cellulase activity quantified by 4-Methylumbelliferyl β-d-cellobioside (4‐MUC). Our findings provide a basis for future research on the enzyme activities of microbes isolated from the digestive tracts of invertebrates and their biocatalytic role in biomass degradation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
World Journal of Microbiology and Biotechnology
ISSN
0959-3993
e-ISSN
1573-0972
Volume of the periodical
39
Issue of the periodical within the volume
7
Country of publishing house
DE - GERMANY
Number of pages
17
Pages from-to
169
UT code for WoS article
000976075800002
EID of the result in the Scopus database
2-s2.0-85154601561