Identification of silk components in the bombycoid moth Andraca theae (Endromidae) reveals three fibroin subunits resembling those of Bombycidae and Sphingidae
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00572250" target="_blank" >RIV/60077344:_____/23:00572250 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/23:43906492
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0022191023000495/pdfft?md5=1611d6411fdd9b4f209abe959882eb5f&pid=1-s2.0-S0022191023000495-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0022191023000495/pdfft?md5=1611d6411fdd9b4f209abe959882eb5f&pid=1-s2.0-S0022191023000495-main.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jinsphys.2023.104523" target="_blank" >10.1016/j.jinsphys.2023.104523</a>
Alternative languages
Result language
angličtina
Original language name
Identification of silk components in the bombycoid moth Andraca theae (Endromidae) reveals three fibroin subunits resembling those of Bombycidae and Sphingidae
Original language description
The silk produced by Lepidoptera caterpillars is a mixture of proteins secreted by the transformed labial glands, the silk glands (SG). The silk fiber consists of insoluble filamentous proteins that form a silk core and are produced in the posterior part of the SG and soluble coat proteins consisting of sericins and various other polypeptides secreted in the middle part of the SG. We constructed a silk gland specific transcriptome of Andraca theae and created a protein database required for peptide mass fingerprinting. We identified major silk components by proteomic analysis of cocoon silk and by searching for homologies with known silk protein sequences from other species. We identified 30 proteins including a heavy chain fibroin, a light chain fibroin and fibrohexamerin (P25) that form the silk core, as well as members of several structural families that form the silk coating. To uncover the evolutionary relationships among silk proteins, we included orthologs of silk genes from several recent genome projects and performed phylogenetic analyses. Our results confirm the recent molecular classification that the family Endromidae appears to be slightly more distant from the family Bombycidae. Our study provides important information on the evolution of silk proteins in the Bombycoidea, which is needed for proper annotation of the proteins and future functional studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Insect Physiology
ISSN
0022-1910
e-ISSN
1879-1611
Volume of the periodical
147
Issue of the periodical within the volume
JUN 01
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
104523
UT code for WoS article
001009321800001
EID of the result in the Scopus database
2-s2.0-85160966695