Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00572378" target="_blank" >RIV/60077344:_____/23:00572378 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/23:00132146 RIV/62157124:16270/23:43881026

Result on the web

<a href="https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011038" target="_blank" >https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pntd.0011038" target="_blank" >10.1371/journal.pntd.0011038</a>

Result language

angličtina

Original language name

Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid

Original language description

Author summaryA potentially fatal disease, neuroangiostrongyliasis, is caused by the rat lungworm (Angiostrongylus cantonensis). The parasite migrates into the spinal cord and brain of accidental hosts, such as humans and dogs, after ingestion of infective larvae, for example by eating snails in garden fruit or vegetables. Recently, an ultrasensitive molecular assay which can detect tiny fragments of the parasite's DNA was developed and has been used to establish a diagnosis. Although this assay outperforms previously developed assays, it requires clean DNA with specialised equipment in a laboratory setting. There is an urgent need for an alternative diagnostic method which is sensitive and portable, for deployment in the field and in the hospitals in remote areas or in low-income countries. The authors developed a fast and portable loop-mediated isothermal amplification (LAMP) assay that compares favourably to the ultra-sensitive PCR assay when tested using cerebrospinal fluid from dogs on the Australian east coast with presumptive neuroangiostrongyliasis. Considering a 'One Health' approach to diagnostics, this assay enables portable emergency diagnostics equally suitable to humans, dogs and wildlife. The newly developed assay will also enable water supplies to be screened, as well as crustaceans and molluscs used as potential food sources, for presence of the parasite.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30303 - Infectious Diseases

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS Neglected Tropical Diseases

ISSN

1935-2735

e-ISSN

1935-2735

Volume of the periodical

17

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

e0011038

UT code for WoS article

000984025300006

EID of the result in the Scopus database

2-s2.0-85159740412