A single cryptomonad cell harbors a complex community of organelles, bacteria, a and selfish elements
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00574484" target="_blank" >RIV/60077344:_____/23:00574484 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17310/23:A2402KYT RIV/60076658:12310/23:43906437
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S096098222300458X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S096098222300458X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cub.2023.04.010" target="_blank" >10.1016/j.cub.2023.04.010</a>
Alternative languages
Result language
angličtina
Original language name
A single cryptomonad cell harbors a complex community of organelles, bacteria, a and selfish elements
Original language description
Symbiosis between prokaryotes and microbial eukaryotes (protists) has broadly impacted both evolution and ecology. Endosymbiosis led to mitochondria and plastids, the latter spreading across the tree of eukary-otes by subsequent rounds of endosymbiosis. Present-day endosymbionts in protists remain both common and diverse, although what function they serve is often unknown. Here, we describe a highly complex com-munity of endosymbionts and a bacteriophage (phage) within a single cryptomonad cell. Cryptomonads are a model for organelle evolution because their secondary plastid retains a relict endosymbiont nucleus, but only one previously unidentified Cryptomonas strain (SAG 25.80) is known to harbor bacterial endosymbionts. We carried out electron microscopy and FISH imaging as well as genomic sequencing on Cryptomonas SAG 25.80, which revealed a stable, complex community even after over 50 years in continuous cultivation. We identified the host strain as Cryptomonas gyropyrenoidosa, and sequenced genomes from its mitochondria, plastid, and nucleomorph (and partially its nucleus), as well as two symbionts, Megaira polyxenophila and Grellia numerosa, and one phage (MAnkyphage) infecting M. polyxenophila. Comparing closely related en-dosymbionts from other hosts revealed similar metabolic and genomic features, with the exception of abun-dant transposons and genome plasticity in M. polyxenophila from Cryptomonas. We found an abundance of eukaryote-interacting genes as well as many toxin-antitoxin systems, including in the MAnkyphage genome that also encodes several eukaryotic-like proteins. Overall, the Cryptomonas cell is an endosymbiotic conglomeration with seven distinct evolving genomes that all show evidence of inter-lineage conflict but nevertheless remain stable, even after more than 4,000 generations in culture.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Biology
ISSN
0960-9822
e-ISSN
1879-0445
Volume of the periodical
33
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
1982-+
UT code for WoS article
001008944500001
EID of the result in the Scopus database
2-s2.0-85159478380