Germline Editing of Drosophila Using CRISPR-Cas9-based Cytosine and Adenine Base Editors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00577609" target="_blank" >RIV/60077344:_____/23:00577609 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/23:43907454
Result on the web
<a href="https://www.liebertpub.com/doi/epub/10.1089/crispr.2023.0026" target="_blank" >https://www.liebertpub.com/doi/epub/10.1089/crispr.2023.0026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/crispr.2023.0026" target="_blank" >10.1089/crispr.2023.0026</a>
Alternative languages
Result language
angličtina
Original language name
Germline Editing of Drosophila Using CRISPR-Cas9-based Cytosine and Adenine Base Editors
Original language description
Target-AID, BE3, and ABE7.10 base editors fused to the catalytically modified Cas9 and xCas9(3.7) were tested for germline editing of the fruit fly Drosophila melanogaster. We developed a guide RNA-expressing construct, white-4gRNA, targeting splice sites in the white gene, an X-chromosome located gene. Using white-4gRNA flies and transgenic lines expressing Target-AID, BE3, and ABE7.10 base editors, we tested the efficiency of stable germline gene editing at three different temperatures. Classical Cas9 generating insertions/deletions by non-homologous end joining served as a reference. Our data indicate that gene editing is most efficient at 28 C, the highest temperature suitable for fruit flies. Finally, we created a new allele of the core circadian clock gene timeless using Target-AID. This base edited mutant allele timSS308-9FL had a disrupted circadian clock with a period of *29 h. The white-4gRNA expressing fly can be used to test new generations of base editors for future applications in Drosophila.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/GA22-10088S" target="_blank" >GA22-10088S: Functional Analysis of Drosophila Timeless Protein</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CRISPR J
ISSN
2573-1599
e-ISSN
2573-1602
Volume of the periodical
6
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
557-569
UT code for WoS article
001130373700001
EID of the result in the Scopus database
2-s2.0-85176465060