Mortality caused by extracellular freezing is associated with fragmentation of nuclear DNA in larval haemocytes of two drosophilid flies

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00577783" target="_blank" >RIV/60077344:_____/23:00577783 - isvavai.cz</a>
Result on the web
<a href="https://journals.biologists.com/jeb/article-pdf/226/21/jeb246456/3244396/jeb246456.pdf" target="_blank" >https://journals.biologists.com/jeb/article-pdf/226/21/jeb246456/3244396/jeb246456.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1242/jeb.246456" target="_blank" >10.1242/jeb.246456</a>

Result language
angličtina
Original language name
Mortality caused by extracellular freezing is associated with fragmentation of nuclear DNA in larval haemocytes of two drosophilid flies
Original language description
The great complexity of extracellular freezing stress, involving mechanical, osmotic, dehydration and chemical perturbations of the cellular milieu, hampers progress in understanding the nature of freezing injury and the mechanisms to cope with it in naturally freeze- tolerant insects. Here, we show that nuclear DNA fragmentation begins to occur in larval haemocytes of two fly species, Chymomyza costata and Drosophila melanogaster, before or at the same time as the sub-zero temperature is reached that causes irreparablenfreezing injury and mortality in freeze-sensitive larval phenotypes. However, when larvae of the freeze-tolerant phenotype (diapausing–cold acclimated–hyperprolinemic) of C. costata were subjected to severe freezing stress in liquid nitrogen, no DNA damage was observed. Artificially increasing the proline concentration in freeze-sensitive larvae of both species by feeding them a proline-enriched diet resulted in a decrease in the proportion of nuclei with fragmented DNA during freezing stress. Our results suggest thatproline accumulated in diapausing C. costata larvae during cold acclimation may contribute to the protection of nuclear DNA against fragmentation associated with freezing stress.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology

Project
<a href="/en/project/GA19-13381S" target="_blank" >GA19-13381S: Cryoprotectants and cryoprotection: assessment of candidate molecules derived from research on freeze-tolerant drosophilid fly.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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