Optimal protein production by a synthetic microbial consortium: coexistence, distribution of labor, and syntrophy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00583797" target="_blank" >RIV/60077344:_____/23:00583797 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s00285-023-01935-3" target="_blank" >https://doi.org/10.1007/s00285-023-01935-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00285-023-01935-3" target="_blank" >10.1007/s00285-023-01935-3</a>

Result language

angličtina

Original language name

Optimal protein production by a synthetic microbial consortium: coexistence, distribution of labor, and syntrophy

Original language description

The bacterium E. coli is widely used to produce recombinant proteins such as growth hormone and insulin. One inconvenience with E. coli cultures is the secretion of acetate through overflow metabolism. Acetate inhibits cell growth and represents a carbon diversion, which results in several negative effects on protein production. One way to overcome this problem is the use of a synthetic consortium of two different E. coli strains, one producing recombinant proteins and one reducing the acetate concentration. In this paper, we study a mathematical model of such a synthetic community in a chemostat where both strains are allowed to produce recombinant proteins. We give necessary and sufficient conditions for the existence of a coexistence equilibrium and show that it is unique. Based on this equilibrium, we define a multi-objective optimization problem for the maximization of two important bioprocess performance metrics, process yield and productivity. Solving numerically this problem, we find the best available trade-offs between the metrics. Under optimal operation of the mixed community, both strains must produce the protein of interest, and not only one (distribution instead of division of labor). Moreover, in this regime acetate secretion by one strain is necessary for the survival of the other (syntrophy). The results thus illustrate how complex multi-level dynamics shape the optimal production of recombinant proteins by synthetic microbial consortia.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10618 - Ecology

Project

<a href="/en/project/EF18_053%2F0016982" target="_blank" >EF18_053/0016982: International mobilities for researchers and administrative employees of Biology Centre</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Mathematical Biology

ISSN

0303-6812

e-ISSN

1432-1416

Volume of the periodical

87

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

36

Pages from-to

23

UT code for WoS article

001021487000002

EID of the result in the Scopus database

2-s2.0-85164154826